CHAITANYA BHARATHI INSTITUTE OF TECHNOLOGY (A)
  • Genetic Engineering and DNA Technology-Biotech-V-Sem-2020-21
    0%
  • Previous
  • Course data
    General
    Announcements
    Genetic Engineering and rDNA Technology Syllabus
    Slip Test I
    Slip Test 2
    Slip test 3
    Slip Test 4
    Assignment 1
    Introduction to Genetic Engineering and rDNA Technology
    Introduction
    Introduction to Genetic Engineeering and rDNA Technology
    UNIT I
    Isolation and purification of DNA Part I
    Isolation and Purification of DNA Part II
    Isolation and Purification of DNA
    Host controlled restriction and modification system
    Host controlled restriction and modification system
    Enzymes used in cloning - Restriction endonuclease
    Enzymes used in cloning - Restriction endonuclease
    Enzymes used in cloning - Restriction endonuclease and DNA Ligase
    Enzymes used in cloning - DNA Ligase
    Enzymes used in cloning - Polymerase
    Enzymes used in cloning - Polymerase
    Enzymes used in cloning - Phosphatase, Kinase and Nuclease
    Enzymes used in cloning - Phosphatase, Kinase and Nuclease
    Restriction mapping and nuclease
    Restriction mapping
    Restriction mapping problems part I
    Restriction mapping problems part II
    Restriction mapping problems
    Blotting techniques – Southern, Northern and Western Blotting
    Blotting techniques – Southern, Northern and Western Blotting
    UNIT II Recordings
    Essential features of cloning vectors, Cloning vectors - Plasmid vectors - pBR 322
    Plasmid vector pUC18, Phage vectors – λZAP, λEMBL4, M13 derived vectors –M13mp18
    Phagemid- Blue script vectors, Cosmid- pJB8, BAC
    Artificial chromosomes - YAC
    Expression vector - pET vectors, Unit - II revision
    UNIT II PPT
    Essential features of cloning vectors, Cloning vectors - Plasmid vectors - pBR 322
    Plasmid vector pUC18, Phage vectors – λZAP, λEMBL4, M13 derived vectors –M13mp18
    Phagemid- Blue script vectors, Cosmid- pJB8, BAC
    Artificial chromosomes - YAC
    Expression vector - pET vectors
    UNIT III RECORDINGS
    PCR – Principle, PCR Methodology
    Designing of primers, Applications of PCR
    Applications of PCR, Multiplex PCR, PCR for site-directed mutagenesis
    RT-PCR, Covid 19 diagnosis by RT-PCR
    Molecular Markers - RFLP, RAPD and AFLP
    UNIT III PPTs
    PCR – Principle, PCR Methodology
    Designing of primers, Applications of PCR
    18. Applications of PCR, Multiplex PCR, PCR for site-directed mutagenesis
    RT-PCR, Covid 19 diagnosis by RT-PCR
    Molecular Markers - RFLP, RAPD and AFLP
    UNIT IV RECORDINGS
    Basic concept of blunt end and cohesive end ligation, homopolymer tailing, use of linkers, adaptors
    Introduction of cloned genes into hosts- Transformation, Transfection, packaging phage DNA In vitro
    Detection of clones with the desired gene part 1
    Detection of clones with the desired gene part 2
    Gene Sequencing - Maxam and Gilbert method
    Gene Sequencing - Sangers Method
    Methods of gene sequencing: Pyrosequencing, automation of DNA sequencing.
    Construction of genomic library and discussion of MId I Key
    Construction of cDNA Library
    UNIT IV PPTs
    Basic concept of blunt end and cohesive end ligation, homopolymer tailing, use of linkers, adaptors
    Introduction of cloned genes into hosts- Transformation, Transfection, packaging phage DNA In vitro
    Detection of clones with the desired gene part 1
    Detection of clones with the desired gene part 2
    Gene Sequencing - Maxam and Gilbert method
    Gene Sequencing - Sangers Method
    Methods of gene sequencing: Pyrosequencing, automation of DNA sequencing.
    Construction of genomic library
    Construction of cDNA Library
    UNIT V RECORDINGS
    High-level expression of proteins in different host systems - E. coli
    High-level expression of proteins in different host systems - yeast
    High-level expression of proteins in different host systems - Insect
    Application of rDNA Technology- Recombinant Insulin and Factor VIII
    Golden rice and Biosaftey guidelines in rDNA research
    Gene therapy - Ex Vivo Gene Therapy - ADA Case Study
    Gene Therapy - In vivo gene therapy
    High-level expression of proteins in different host systems - Mammals
    UNIT V PPTs
    High-level expression of proteins in different host systems - E. coli
    High-level expression of proteins in different host systems - yeast
    High-level expression of proteins in different host systems - Insect
    Application of rDNA Technology- Recombinant Insulin and Factor VIII
    Golden rice and Biosaftey guidelines in rDNA research
    Gene Therapy - Ex Vivo Gene Therapy - ADA case Study
    Gene Therapy - In vivo gene therapy
    High-level expression of proteins in different host systems - Mammals
    GE NOTES
    GE UNIT II NOTES
    Genetic Engineeering Lab
    Introduction of Genetic Engineering Lab
  • Next
  CHAITANYA BHARATHI INSTITUTE OF TECHNOLOGY (A)
    • Log in
      Forgot Password?
    Genetic Engineering and DNA Technology-Biotech-V-Sem-2020-21
    Home
    Calendar
    Skip to main content

    Course info

    1. Home
    2. Courses
    3. Biotechnology
    4. AY 2020-21
    5. V Semester
    6. Genetic Engineering and DNA Technology-Biotech-V-Sem-2020-21
    7. Summary

    Genetic Engineering and DNA Technology-Biotech-V-Sem-2020-21

    • Teacher: Dr.V.Aruna Assistant Professor
    Skill Level: Beginner

    Follow Us

    Chaitanya Bharathi Institute of Technology(Autonomous), Hyderabad
    You are currently using guest access (Log in)
    Get the mobile app