CHAITANYA BHARATHI INSTITUTE OF TECHNOLOGY (A)
  • Biotech- Genetic Engineering and rDNA Technology -V-2021-22
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  • Course data
    General
    Announcements
    Syllabus and Assessments
    Genetic Engineering and rDNA Technology Syllabus
    Slip Test 1
    GE UNIT I Recordings
    AY2021-UG-Sem-5-18BTC17-GE-VA-20210805-1-Introduction to Genetic Engineering and rDNA technology
    AY2021-UG-Sem-5-18BTC17-GE-VA-20210806-2-Isolation and Purification of DNA-Total DNA
    AY2021-UG-Sem-5-18BTC17-GE-VA-20210809-3-Isolation and Purification of DNA-Plasmid and Phage DNA
    AY2021-UG-Sem-5-18BTC17-GE-VA-20210812-4-Host Controlled restriction and modification
    AY2021-UG-Sem-5-18BTC17-GE-VA-20210813-5-Enzymes used in cloning - Restriction endonuclease
    AY2021-UG-Sem-5-18BTC17-GE-VA-20210816-6-Enzymes used in cloning - DNA Ligase
    AY2021-UG-Sem-5-18BTC17-GE-VA-20210819-7-Enzymes used in cloning - Polymerase
    AY2021-UG-Sem-5-18BTC17-GE-VA-20210819-8-Enzymes used in cloning - Phosphatases, Kinases and Nuclease
    AY2021-UG-Sem-5-18BTC17-GE-VA-20210823-9-Restriction Mapping
    AY2021-UG-Sem-5-18BTC17-GE-VA-20210826-10- Blotting Technique - Southern, Northern and Western Blotting
    GE UNIT I PPTs
    1. Introduction to Genetic Engineering and rDNA Technology
    2. Isolation and purification of DNA
    3. Host Controlled restriction and modification
    4. Enzymes used in cloning - Restriction endonuclease
    5. Enzymes used in cloning - DNA Ligase
    6. Enzymes used in cloning - Polymerase
    7. Enzymes used in cloning - Phosphatase, Kinase and Nuclease
    8. Restriction Mapping
    9. Restriction Mapping Problems
    10. Blotting techniques – Southern, Northern and Western Blotting
    UNIT II Recordings
    AY2021-UG-Sem-5-18BTC17-GE-VA-20210827-11-Essential features of cloning vectors; Cloning vectors - Plasmid vectors - pBR 322
    AY2021-UG-Sem-5-18BTC17-GE-VA-20210830-12-Plasmid vectors - pUC18, Phage vectors – λZAP, λEMBL4
    AY2021-UG-Sem-5-18BTC17-GE-VA-20210901-13-M13 derived vectors, Phagmids, Cosmids, BAC
    AY2021-UG-Sem-5-18BTC17-GE-VA-20210902-14-Cosmids, YAC
    AY2021-UG-Sem-5-18BTC17-GE-VA-20210903-15-Expression vectors - pET vectors
    UNIT II PPTs
    11. Essential features of cloning vectors, Cloning vectors - Plasmid vectors - pBR 322
    12. Plasmid vector pUC18, Phage vectors – λZAP, λEMBL4, M13 derived Vectors
    13. Phagemid- Blue script vectors, Cosmid- pJB8, BAC
    14. Artificial chromosomes - YAC
    15. Expression vectors - pET vectors
    UNIT III Recordings
    AY2021-UG-Sem-5-18BTC17-GE-VA-20210906-16- PCR – Principle
    AY2021-UG-Sem-5-18BTC17-GE-VA-20210909-17- PCR – Methodology, Primer Design and Applications
    AY2021-UG-Sem-5-18BTC17-GE-VA-20211001-18- RT-PCR
    AY2021-UG-Sem-5-18BTC17-GE-VA-20211004-19- Multiplex PCR, PCR for Site directed Mutagenesis
    UNIT III PPTs
    16. PCR – Principle, PCR Methodology
    17. Designing of primers, Applications of PCR
    18. Applications of PCR, Multiplex PCR, PCR for site-directed mutagenesis
    19. RT PCR
    20. Molecular Markers
    UNIT IV Recordings
    AY2021-UG-Sem-5-18BTC17-GE-VA-20211005-20-Basic concept of blunt end and cohesive end ligation, homopolymer tailing, use of linkers, adaptors
    AY2021-UG-Sem-5-18BTC17-GE-VA-20211008-21, 22-Introduction of cloned genes into hosts- Transformation, Transfection, packaging phage DNA In vitro, Detection of clone
    AY2021-UG-Sem-5-18BTC17-GE-VA-20211011-23-Detection of clone with desired gene
    AY2021-UG-Sem-5-18BTC17-GE-VA-20211012-24-DNA Sequencing
    AY2021-UG-Sem-5-18BTC17-GE-VA-20211018-25-DNA Sequencing
    AY2021-UG-Sem-5-18BTC17-GE-VA-20211022-26-Construction of Genomic Library
    UNIT IV PPTs
    21. Basic concept of blunt end and cohesive end ligation, homopolymer tailing, use of linkers, adaptors
    22. Introduction of cloned genes into hosts- Transformation, Transfection, packaging phage DNA In vitro
    23. Detection of clones with the desired gene part 1
    23. Detection of clones with the desired gene part 2
    24. Gene Sequencing - Maxam and Gilbert method
    25. Gene Sequencing - Sangers Method
    26. Gene Sequencing - Pyrosequencing, automation of DNA sequencing.
    27. Genomic Library
    28. Construction of cDNA Library
    UNIT V PPTs
    29. High-level expression of proteins in different host systems - E. coli
    30. High-level expression of proteins in different host systems - Yeast
    31. High-level expression of proteins in different host systems - Insect
    32. High-level expression of proteins in different host systems - Mammals
    33. Application of rDNA Technology- Recombinant Insulin and Factor VIII
    34. Golden rice and Biosaftey guidelines in rDNA research
    35. Gene therapy - Ex Vivo Gene Therapy - ADA Case Study
    36. Gene Therapy - In vivo gene therapy
    Topic 12
    Question bank
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    Biotech- Genetic Engineering and rDNA Technology -V-2021-22
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    6. Biotech- Genetic Engineering and rDNA Technology -V-2021-22
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    Biotech- Genetic Engineering and rDNA Technology -V-2021-22

    • Teacher: Dr.V.Aruna Assistant Professor
    Skill Level: Beginner

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